About the project
Project Manager
Bessembayeva Lyailya
master of engineering
Scopus Author ID: 57205304219
Researcher ID: AGV-7724-2022
ORCID: 0000-0002-6045-7462
Relevance
Bovine mastitis remains one of the most widespread and economically significant diseases in the dairy industry worldwide, including the Republic of Kazakhstan. The disease leads to reduced milk yield, deterioration of milk quality, premature culling of animals, and substantial financial losses for farms. Microorganisms of the genus Staphylococcus spp. play a crucial role in the etiology of mastitis, being characterized by high virulence and a strong ability to form biofilms.
In recent years, the increasing prevalence of methicillin-resistant (MRSA), vancomycin-resistant (VRSA), and multidrug-resistant staphylococcal strains has become a serious concern, significantly reducing the effectiveness of conventional antibiotic therapy. Biofilm formation further enhances pathogen resistance to antimicrobial agents and host immune defenses, contributing to the chronic and recurrent nature of inflammatory processes in the mammary gland.
The widespread and often uncontrolled use of antibiotics in veterinary practice accelerates the spread of antimicrobial resistance and results in antibiotic residues in milk, posing a threat to food safety and public health. Therefore, the search for effective non-antibiotic alternative treatments for mastitis is a priority in modern veterinary science and aligns with the global “One Health” concept.
Plant extracts rich in biologically active compounds have demonstrated strong antimicrobial and antibiofilm activities and are considered a promising approach for developing safe and environmentally friendly therapeutic agents. The rich botanical diversity of Kazakhstan provides unique opportunities for identifying and scientifically validating phytotherapeutic products effective against antibiotic-resistant mastitis pathogens.
In this context, the development of plant extract-based formulations aimed at eradicating biofilms formed by MRSA, VRSA, and multidrug-resistant staphylococci is scientifically justified, socially important, and economically viable. The implementation of this project will contribute to reducing antibiotic dependence in dairy farming, improving mastitis treatment outcomes, enhancing milk quality, and addressing the global challenge of antimicrobial resistance.
Purpose
Development of drugs based on plant extracts against cow mastitis, study of their antimicrobial, anti-biofilm effects against antibiotic-resistant Staphylococcus spp.
Expected and achieved results
1. Monitoring the spread of cow mastitis caused by MRSA and VRSA, multidrug-resistant strains of Staphylococcus spp. in farms in the south and southeast of Kazakhstan.
2. Phenotypic and genotypic characterization of MRSA and VRSA, and multidrug-resistant Staphylococcus spp. strains.
3. Determination of biofilm formation ability of isolated cultures of Staphylococcus spp. and assessment of the degree of their resistance to antibiotics in comparison with planktonic forms.
4. Selection of components for polyphyte formulation from medicinal plants with pronounced antimicrobial and antibiofilm properties.
5. Determination of technology for obtaining plant extracts for polyphyte formulation.
6. Determination of minimum biofilm inhibition concentration and determination of minimum biofilm eradication concentration of MRSA and VRSA, and multidrug-resistant Staphylococcus spp.
7. Assessment of therapeutic efficacy of the developed preparation based on polyfit formula in mastitis of cows in conditions of farms of Almaty region.
Information for potential users
A substantial proportion of economic losses in dairy farming is associated with prolonged treatment of mastitis and reduced cow productivity, particularly in infections caused by antibiotic-resistant microorganisms. The spread of methicillin-resistant and multidrug-resistant Staphylococcus spp. strains significantly limits the effectiveness of conventional veterinary drugs and increases farm expenditures on therapy and animal culling.
The development of a domestic anti-mastitis formulation based on plant extracts, active against antibiotic-resistant and biofilm-forming pathogens, will reduce dependence on antibiotics, improve treatment outcomes, and decrease financial losses in dairy enterprises.
The implementation of the developed technology will contribute to herd preservation, increased productivity, and the production of high-quality milk without risks associated with antibiotic residues.
Scientific adviser
Kirkimbayeva Zhumagul
Doctor of Veterinary Sciences, Professor
Scopus Author ID: 56273059600
ORCID:0000-0003-3873-9049